The A433 Fosseway, Kemble, will close for approximately four nights excluding weekends between 7pm and 7am.
Works are expected to start on Thursday, June 11, and end on Tuesday, June 16.
A spokesman for Gloucestershire Highways said: “Due to the ongoing Public Health emergency being experienced throughout the UK, we will no longer be letter dropping prior to road closures at resurfacing sites.
“We will continue to perform resurfacing whilst we have available resource and material, and will endeavour to put up advance notice boards on these sites, however, the situation is changing regularly and this may result in works being postponed or cancelled at short notice.”
The closure and diversion route will be clearly indicated by traffic signs.