Poundland in Stroud is closing down this weekend.
The store will close on Sunday to make way for a new £6.5m medical centre.
A spokesman for Poundland said: “Our lease on the Stroud store is expiring and the landlord has other plans for the property.
“We’re sad to be going and it goes without saying that when we close a store we always work hard to look after colleagues directly affected.”
The Poundland premises, formerly Woolworths, in King Street will be refurbished and partially rebuilt to create the new surgery.
The project will create a new £6.5m town centre location for two GP surgeries as well as a pharmacy and additional space for a range of other complementary health and service providers.
Construction work is due to start in the first week of August and the main building work on the new surgery is scheduled to be completed in August 2022, ready for opening in the autumn.
The medical centre will be home to Locking Hill Surgery and Stroud Valleys Family Practice.