Two men have been arrested on suspicion of causing death by dangerous driving.
The incident happened at around 10.50pm last night (May 19) in Ermin Street in Brockworth, close to the Invista Textile Factory.
The rider of the motorcycle, an 18-year-old man from the Brockworth area, sustained fatal injuries and was pronounced dead at the scene.
His next of kin have been informed and are being supported by specially trained officers.
A 36-year-old man from Tetbury and a 32-year-old man from Brockworth have been arrested on suspicion of causing death by dangerous driving.
They remain in police custody at this time.
The road currently remains closed between the junctions of Vicarage Lane and Hucclecote Road and is expected to remain shut for most of the morning.
Officers would like speak to anyone who may have witnessed the collision or who may have relevant dashcam footage.
Anyone with information is asked to contact Gloucestershire Constabulary through the website, quoting incident 538 of May 19.